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Home > Product > ELISA Kit > Rat Aggrecan Core Protein, ACAN ELISA Kit

Rat Aggrecan Core Protein, ACAN ELISA Kit

Rat Aggrecan Core Protein, ACAN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.74ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 5.74ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Aggrecan Core Protein

Code

E1852Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.74ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

UniProt accession

MASS(Da)

221,118

GeneID

58968

Synonyms

ACAN;AGCAN;Aggrecan 1;Aggrecan core protein;Cartilage-specific proteoglycan core protein;CSPCP;CSPG1;MSK16;SEDK;

Gene names

ACAN

Research area

Signal Transduction

Target protein

ACAN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1852Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.481 2.48 2.435
2.479
600ng/L 1.526 1.448 1.403
1.37
300ng/L 0.809 0.773 0.728
0.736
150ng/L 0.409 0.404 0.359
0.399
75ng/L 0.253 0.234 0.189
0.215
0ng/L 0.056 0.045 0
0.034

Precision

We measured random samples of E1852Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 320.4 16.41 5.1
Intra-Assay 2 18 653.6 20.32 3.1
Intra-Assay 3 18 254.6 12.2 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E1852Ra

    $320.00

  • 96T

    E1852Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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