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Home > Product > ELISA Kit > Human Toll-like Receptor 9, TLR-9 ELISA Kit

Human Toll-like Receptor 9, TLR-9 ELISA Kit

Human Toll-like Receptor 9, TLR-9 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 2.53ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 2.53ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Toll-Like Receptor 9

Code

E0356Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

2.53ng/L

Detection range

5-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed: 11564765, PubMed: 17932028). Controls lymphocyte response to Helicobacter infection (By similarity). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed: 23857366).

UniProt accession

MASS(Da)

115,860

GeneID

54106

Synonyms

CD antigen CD289;TLR 9;TLR9;Toll-like receptor 9

Gene names

TLR9

Research area

Immunology; Signal Transduction

Target protein

TLR9

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0356Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.142 2.092 2.039
2.042
400ng/L 1.423 1.428 1.375
1.433
200ng/L 0.747 0.75 0.697
0.754
100ng/L 0.436 0.374 0.321
0.313
50ng/L 0.188 0.188 0.135
0.188
0ng/L 0.051 0.053 0
0.055

Precision

We measured random samples of E0356Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 114.4 4.08 3.6
Intra-Assay 2 18 592.6 22.73 3.8
Intra-Assay 3 18 285.1 13.58 4.8

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 96T

    E0356Hu

    $458.00

  • 48T

    E0356Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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