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Home > Product > ELISA Kit > Rat Coagulation Factor 7, F7 ELISA Kit

Rat Coagulation Factor 7, F7 ELISA Kit

Rat Coagulation Factor 7, F7 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.147ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.147ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Coagulation Factor 7

Code

E1914Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.147ng/mL

Detection range

0.3-90ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).

UniProt accession

MASS(Da)

50399

GeneID

260320

Synonyms

Coagulation factor VII;F7;Factor VII heavy chain;Factor VII light chain;Serum prothrombin conversion accelerator

Gene names

F7

Research area

Blood Coagulation

Target protein

F7

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1914Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/mL 1.949 2.121 2.096
2.294
24ng/mL 1.56 1.507 1.481
1.454
12ng/mL 0.618 0.634 0.608
0.65
6ng/mL 0.456 0.414 0.388
0.372
3ng/mL 0.157 0.18 0.154
0.202
0ng/mL 0.024 0.026 0
0.027

Precision

We measured random samples of E1914Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.8 0.32 4.8
Intra-Assay 2 18 19.1 1.03 5.4
Intra-Assay 3 18 23.2 0.73 3.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E1914Ra

    $320.00

  • 96T

    E1914Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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