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Home > Product > ELISA Kit > Human Toll-like Receptor 1, TLR1 ELISA Kit

Human Toll-like Receptor 1, TLR1 ELISA Kit

Human Toll-like Receptor 1, TLR1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.09ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.09ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Toll-Like Receptor 1

Code

E0363Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.09ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed: 21078852). Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed: 16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

UniProt accession

MASS(Da)

90,291

GeneID

7096

Synonyms

CD antigen CD281;TIL;TLR 1;TLR1;Toll/interleukin-1 receptor-like protein;Toll-like receptor 1

Gene names

TLR1

Research area

Immunology

Target protein

TLR1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0363Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.246 2.301 2.271
2.355
20ng/ml 1.363 1.408 1.379
1.454
10ng/ml 0.603 0.658 0.629
0.713
5ng/ml 0.385 0.348 0.319
0.311
2.5ng/ml 0.154 0.139 0.11
0.125
0ng/ml 0.037 0.03 0
0.022

Precision

We measured random samples of E0363Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 18.6 0.66 3.6
Intra-Assay 2 18 6.7 0.28 4.2
Intra-Assay 3 18 11.4 0.45 4

Protocols

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  • Size

    Code

    Price

  • 96T

    E0363Hu

    $458.00

  • 48T

    E0363Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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