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Home > Product > ELISA Kit > Rat Hypoxia-inducible Factor 3-alpha, HIF3A ELISA Kit

Rat Hypoxia-inducible Factor 3-alpha, HIF3A ELISA Kit

Rat Hypoxia-inducible Factor 3-alpha, HIF3A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.22ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 7.22ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Hypoxia-Inducible Factor 3-Alpha

Code

E2012Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.22ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a transcriptional regulator in adaptive response to low oxygen tension. Attenuates the ability of transcription factor HIF1A, EPAS1 and the HIF1A-ARNT complex to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis. May act as a tumor suppressor.

UniProt accession

MASS(Da)

72,888

GeneID

64345

Synonyms

HIF 3A;HIF3A;HIF3-alpha;HIF-3-alpha;HIF3-alpha-1;Hypoxia-inducible factor 3-alpha;IPAS

Gene names

HIF3A

Research area

Cardiovascular

Target protein

HIF3A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2012Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.22 2.315 2.24
2.411
800ng/L 1.477 1.416 1.34
1.355
400ng/L 0.899 0.811 0.736
0.723
200ng/L 0.409 0.443 0.367
0.476
100ng/L 0.255 0.243 0.168
0.232
0ng/L 0.074 0.075 0
0.076

Precision

We measured random samples of E2012Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1074.8 37.65 3.5
Intra-Assay 2 18 427.6 24.74 5.8
Intra-Assay 3 18 207.7 12.59 6.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E2012Ra

    $320.00

  • 96T

    E2012Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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