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Home > Product > ELISA Kit > Rat Natriuretic Peptides B, NPPB ELISA Kit

Rat Natriuretic Peptides B, NPPB ELISA Kit

Rat Natriuretic Peptides B, NPPB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.27ng/L

Detection range: 5-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 2.27ng/L

Detection range: 5-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Natriuretic Peptides B

Code

E2105Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.27ng/L

Detection range

5-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

[Brain natriuretic Peptide 45]: Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed: 2525380, PubMed: 9252368). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (By similarity). Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis (PubMed: 2525380, PubMed: 9252368).

UniProt accession

MASS(Da)

13,656

GeneID

25105

Synonyms

5 kDa cardiac natriuretic Peptide;BNP;BNP-45;Brain natriuretic factor prohormone;Brain natriuretic Peptide;Brain natriuretic Peptide 45;Gamma-brain natriuretic Peptide;Iso-ANP;Natriuretic Peptides B;NPPB;preproBNP;proBNP

Gene names

NPPB

Research area

Cardiovascular

Target protein

NPPB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2105Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.094 2.17 2.123
2.247
120ng/L 1.758 1.853 1.805
1.947
60ng/L 1.12 1.179 1.131
1.238
30ng/L 0.707 0.722 0.674
0.737
15ng/L 0.349 0.377 0.33
0.405
0ng/L 0.066 0.047 0
0.029

Precision

We measured random samples of E2105Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 59.7 4 6.7
Intra-Assay 2 18 94.9 5.12 5.4
Intra-Assay 3 18 22.9 1.33 5.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E2105Ra

    $320.00

  • 96T

    E2105Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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