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Home > Product > ELISA Kit > Rat Leukocyte Surface Antigen CD47, CD47 ELISA Kit

Rat Leukocyte Surface Antigen CD47, CD47 ELISA Kit

Rat Leukocyte Surface Antigen CD47, CD47 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.18ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 3.18ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Leukocyte Surface Antigen CD47

Code

E2131Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.18ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus.

UniProt accession

MASS(Da)

32,995

GeneID

29364

Synonyms

CD 47;CD antigen CD47;CD47;IAP;Integrin-associated protein;Leukocyte surface antigen CD47

Gene names

CD47

Research area

Immunology; Cancer

Target protein

CD47

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2131Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 1.714 1.842 1.794
1.97
400ng/L 1.074 1.082 1.034
1.089
200ng/L 0.557 0.501 0.453
0.445
100ng/L 0.186 0.167 0.119
0.149
50ng/L 0.086 0.118 0.07
0.15
0ng/L 0.063 0.048 0
0.033

Precision

We measured random samples of E2131Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 501.2 16.8 3.4
Intra-Assay 2 18 307.3 10.89 3.5
Intra-Assay 3 18 178.4 5.96 3.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E2131Ra

    $320.00

  • 96T

    E2131Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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