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Home > Product > ELISA Kit > Rat Macrophage Metalloelastase, MMP12 ELISA Kit

Rat Macrophage Metalloelastase, MMP12 ELISA Kit

Rat Macrophage Metalloelastase, MMP12 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.139ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.139ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Macrophage Metalloelastase

Code

E2139Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.139ng/mL

Detection range

0.3-90ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity).

UniProt accession

MASS(Da)

53,738

GeneID

117033

Synonyms

Macrophage metalloelastase;Matrix Metalloproteinase-12;MME;MMP 12;MMP12;MMP-12

Gene names

MMP12

Research area

Others

Target protein

MMP12

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2139Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/mL 2.191 2.253 2.223
2.314
24ng/mL 1.62 1.632 1.602
1.644
12ng/mL 0.983 1.028 0.999
1.073
6ng/mL 0.677 0.66 0.63
0.643
3ng/mL 0.445 0.453 0.423
0.461
0ng/mL 0.031 0.03 0
0.029

Precision

We measured random samples of E2139Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 16.8 0.67 4
Intra-Assay 2 18 8.9 0.54 6.1
Intra-Assay 3 18 5.2 0.35 6.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E2139Ra

    $320.00

  • 96T

    E2139Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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