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Home > Product > ELISA Kit > Rat Multidrug Resistance-associated Protein 1, ABCC1 ELISA Kit

Rat Multidrug Resistance-associated Protein 1, ABCC1 ELISA Kit

Rat Multidrug Resistance-associated Protein 1, ABCC1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.45ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 5.45ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Multidrug Resistance-Associated Protein 1

Code

E2156Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.45ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency (PubMed: 15129170). Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation (PubMed: 17050692). Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells (By similarity).

UniProt accession

MASS(Da)

171,493

GeneID

24565

Synonyms

ABCC 1;ABCC1;ATP-binding cassette sub-family C member 1;Glutathione-S-conjugate-translocating ATPase ABCC1;Leukotriene C(4 transporter;LTC4 transporter;Multidrug resistance-associated protein 1

Gene names

ABCC1

Research area

Signal Transduction; Cancer; Cardiovascular; Metabolism

Target protein

ABCC1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2156Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.352 2.384 2.332
2.415
600ng/L 1.407 1.434 1.383
1.461
300ng/L 0.71 0.711 0.66
0.712
150ng/L 0.518 0.494 0.443
0.47
75ng/L 0.303 0.316 0.264
0.328
0ng/L 0.052 0.052 0
0.051

Precision

We measured random samples of E2156Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 731.6 20.57 2.8
Intra-Assay 2 18 425.2 25.79 6.1
Intra-Assay 3 18 273.1 19.89 7.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E2156Ra

    $320.00

  • 96T

    E2156Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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