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Home > Product > ELISA Kit > Human B-cell Linker Protein, BLNK ELISA Kit

Human B-cell Linker Protein, BLNK ELISA Kit

Human B-cell Linker Protein, BLNK ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.18ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.18ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

B-Cell Linker Protein

Code

E0385Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.18ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

UniProt accession

MASS(Da)

50,466

GeneID

29760

Synonyms

B-cell adapter containing a SH2 domain protein;B-cell adapter containing a Src homology 2 domain protein;B-cell linker protein;BLNK;Cytoplasmic adapter protein;SLP-65;Src homology 2 domain-containing leukocyte protein of 65 kDa

Gene names

BLNK

Research area

Others

Target protein

BLNK

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0385Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.151 2.209 2.193
2.266
16ng/ml 1.235 1.261 1.245
1.287
8ng/ml 0.772 0.755 0.739
0.739
4ng/ml 0.348 0.376 0.36
0.404
2ng/ml 0.209 0.169 0.153
0.129
0ng/ml 0.016 0.016 0
0.017

Precision

We measured random samples of E0385Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 22.9 0.72 3.1
Intra-Assay 2 18 5.7 0.33 5.8
Intra-Assay 3 18 10.7 0.55 5.1

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 96T

    E0385Hu

    $458.00

  • 48T

    E0385Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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