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Home > Product > ELISA Kit > Rat Pituitary Adenylate Cyclase-activating Polypeptide, ADCYAP1 ELISA Kit

Rat Pituitary Adenylate Cyclase-activating Polypeptide, ADCYAP1 ELISA Kit

Rat Pituitary Adenylate Cyclase-activating Polypeptide, ADCYAP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.32ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 5.32ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Pituitary Adenylate Cyclase-Activating Polypeptide

Code

E2197Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.32ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells (By similarity). Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (PubMed: 18198219). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (By similarity).

UniProt accession

MASS(Da)

19,557

GeneID

24166

Synonyms

ADCYAP 1;ADCYAP1;PACAP;PACAP27;PACAP-27;PACAP38;PACAP-38;PACAP-related Peptide;Pituitary adenylate cyclase-activating polypeptide;Pituitary adenylate cyclase-activating polypeptide 27;Pituitary adenylate cyclase-activating polypeptide 38;PRP-48

Gene names

ADCYAP1

Research area

Neuroscience

Target protein

ADCYAP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2197Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.247 2.156 2.096
2.065
600ng/L 1.463 1.421 1.361
1.379
300ng/L 0.732 0.756 0.697
0.781
150ng/L 0.509 0.455 0.395
0.401
75ng/L 0.24 0.267 0.207
0.294
0ng/L 0.048 0.06 0
0.071

Precision

We measured random samples of E2197Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 164.8 8.71 5.3
Intra-Assay 2 18 333.8 15.61 4.7
Intra-Assay 3 18 607.8 22.18 3.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E2197Ra

    $320.00

  • 96T

    E2197Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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