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Home > Product > ELISA Kit > Human Protein Phosphatase 1 Regulatory Subunit 1A, PPP1R1A ELISA Kit

Human Protein Phosphatase 1 Regulatory Subunit 1A, PPP1R1A ELISA Kit

Human Protein Phosphatase 1 Regulatory Subunit 1A, PPP1R1A ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 7.17ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 7.17ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein Phosphatase 1 Regulatory Subunit 1A

Code

E0402Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

7.17ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.

UniProt accession

MASS(Da)

19,011

GeneID

5502

Synonyms

I-1;IPP-1;PPP1R1A;Protein phosphatase 1 regulatory subunit 1A;Protein phosphatase inhibitor 1

Gene names

PPP1R1A

Research area

Others

Target protein

PPP1R1A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0402Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.265 2.321 2.245
2.376
800ng/L 1.248 1.277 1.201
1.305
400ng/L 0.721 0.713 0.638
0.706
200ng/L 0.495 0.474 0.399
0.453
100ng/L 0.27 0.274 0.198
0.277
0ng/L 0.072 0.076 0
0.079

Precision

We measured random samples of E0402Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1078.3 24.9 2.3
Intra-Assay 2 18 241.7 17.03 7
Intra-Assay 3 18 594.5 32.45 5.5

Protocols

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  • Size

    Code

    Price

  • 96T

    E0402Hu

    $458.00

  • 48T

    E0402Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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