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Home > Product > ELISA Kit > Rat Thyroxine, T4 ELISA Kit

Rat Thyroxine, T4 ELISA Kit

Rat Thyroxine, T4 ELISA Kit

Product Summary

Size: 96 Test

Sensitivity: 1.25ng/ml

Detection range: 2 - 600ng/ml

Sample type: Serum, plasma, cell culture supernatant

Reactive with: Rat

Product Summary

Size: 96 Test

Sensitivity: 1.25ng/ml

Detection range: 2 - 600ng/ml

Sample type: Serum, plasma, cell culture supernatant

Reactive with: Rat

Product overview

Full product name

Thyroxine

Size

96 Test

Sensitivity

1.25ng/ml

Detection range

2 - 600ng/ml

Sample type

Serum, plasma, cell culture supernatant

Code

EA0027Ra

Assay type

Competitive

Species

Rat

Storage

2-8ºC

Assay time

2h 30m

Background

Thyroid hormones are two hormones produced and released by the thyroid gland, namely triiodothyronine (T3) and thyroxine (T4). They are tyrosine-based hormones that are primarily responsible for regulation of metabolism. T3 and T4 are partially composed of iodine. A deficiency of iodine leads to decreased production of T3 and T4, enlarges the thyroid tissue and will cause the disease known as simple goitre. The major form of thyroid hormone in the blood is thyroxine (T4), which has a longer half-life than T3. In humans, the ratio of T4 to T3 released into the blood is approximately 14:1. T4 is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5′-iodinase). These are further processed by decarboxylation and deiodination to produce iodothyronamine (T1a) and thyronamine (T0a). All three isoforms of the deiodinases are selenium-containing enzymes, thus dietary selenium is essential for T3 production.

Synonyms

T4;Thyroxine

Research area

Signal Transduction

Target protein

T4

Components

Components Quantity
Pre-coated plate 12 * 8 well strips x 1
The standard, lyophilized 2 vials
Standard/Sample diluent 6ml x 1 vial
Biotinylated antigen, lyophilized 1 vial
Avidin-HRP concentrate 100μl x 1 vial
Biotinylated antigen diluent 6ml x 1 vials
Avidin HRP diluent 5.9ml x 1 vials
Substrate solution A 6ml x1 vial
Substrate solution B 6ml x1 vial
Stop solution 6ml x1 vial
Wash buffer concentrate (25x) 20ml x 1 vial

Protocol data

These standard curves of EA0027Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected

Precision

We measured random samples of EA0027Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

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  • Size

    Code

    Price

  • 96 Test

    EA0027Ra

    $458.00

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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