Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Myogenin, MYOG ELISA Kit

Human Myogenin, MYOG ELISA Kit

Human Myogenin, MYOG ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 8.02ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 8.02ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Myogenin

Code

E0421Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

8.02ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).

UniProt accession

MASS(Da)

25,037

GeneID

4656

Synonyms

bHLHc3;Class C basic helix-loop-helix protein 3;Myf-4;MYOG;Myogenic factor 4;Myogenin

Gene names

MYOG

Research area

Others

Target protein

MYOG

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0421Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.676 1.798 1.759
1.919
800ng/L 0.939 1.035 0.997
1.132
400ng/L 0.426 0.421 0.383
0.417
200ng/L 0.158 0.158 0.12
0.157
100ng/L 0.118 0.103 0.065
0.088
0ng/L 0.023 0.038 0
0.053

Precision

We measured random samples of E0421Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1338.3 51.22 3.8
Intra-Assay 2 18 378.1 12.27 3.2
Intra-Assay 3 18 657.6 22.03 3.3

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 96T

    E0421Hu

    $458.00

  • 48T

    E0421Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up