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Home > Product > ELISA Kit > Human Dickkopf-1, DKK1 ELISA Kit

Human Dickkopf-1, DKK1 ELISA Kit

Human Dickkopf-1, DKK1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.26ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.26ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dickkopf-1

Code

E0630Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.26ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed: 22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed: 17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).

UniProt accession

MASS(Da)

28,672

GeneID

22943

Synonyms

Dickkopf-1;Dickkopf-related protein 1;DKK 1;DKK1;Dkk-1;hDkk-1;SK

Gene names

DKK1

Research area

Cardiovascular

Target protein

DKK1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0630Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/ml 2.03 2.101 2.048
2.173
40ng/ml 1.871 1.797 1.744
1.724
20ng/ml 1.15 1.159 1.106
1.168
10ng/ml 0.758 0.735 0.681
0.712
5ng/ml 0.373 0.361 0.308
0.35
0ng/ml 0.042 0.053 0
0.065

Precision

We measured random samples of E0630Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 11 0.69 6.2
Intra-Assay 2 18 15.9 0.94 5.9
Intra-Assay 3 18 35 2 5.7

Protocols

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  • Size

    Code

    Price

  • 96T

    E0630Hu

    $458.00

  • 48T

    E0630Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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