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Home > Product > ELISA Kit > Human Mothers Against Decapentaplegic Homolog 7, SMAD7 ELISA Kit

Human Mothers Against Decapentaplegic Homolog 7, SMAD7 ELISA Kit

Human Mothers Against Decapentaplegic Homolog 7, SMAD7 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Mothers Against Decapentaplegic Homolog 7

Code

E0639Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.022ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

UniProt accession

MASS(Da)

46,426

GeneID

4092

Synonyms

hSMAD7;MAD homolog 7;MAD homolog 8;Mothers against decapentaplegic homolog 7;Mothers against decapentaplegic homolog 8;Mothers against DPP homolog 7;Mothers against DPP homolog 8;SMAD 7;SMAD family member 7;SMAD7

Gene names

SMAD7

Research area

Signal Transduction; Epigenetics and Nuclear Signaling

Target protein

SMAD7

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0639Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.33 2.171 2.108
2.013
6ng/ml 1.299 1.318 1.255
1.337
3ng/ml 0.722 0.81 0.747
0.899
1.5ng/ml 0.549 0.545 0.482
0.542
0.75ng/ml 0.272 0.286 0.222
0.299
0ng/ml 0.064 0.063 0
0.063

Precision

We measured random samples of E0639Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.6 0.24 6.7
Intra-Assay 2 18 7.7 0.25 3.3
Intra-Assay 3 18 1.2 0.07 6.3

Protocols

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  • Size

    Code

    Price

  • 96T

    E0639Hu

    $458.00

  • 48T

    E0639Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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