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Home > Product > ELISA Kit > Human Signal Transducer and Activator of Transcription 3, STAT3 ELISA Kit

Human Signal Transducer and Activator of Transcription 3, STAT3 ELISA Kit

Human Signal Transducer and Activator of Transcription 3, STAT3 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.05ng/ml

Detection range: 0.1-40.ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.05ng/ml

Detection range: 0.1-40.ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Signal Transducer and Activator of Transcription 3

Code

E0650Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.05ng/ml

Detection range

0.1-40.ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed: 10688651, PubMed: 12359225, PubMed: 12873986, PubMed: 15194700, PubMed: 17344214, PubMed: 18242580, PubMed: 23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed: 17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed: 12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed: 12359225). Activated by IL31 through IL31RA (PubMed: 15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed: 28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed: 17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed: 18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed: 23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity).

UniProt accession

MASS(Da)

88,068

GeneID

6774

Synonyms

Acute-phase response factor;Signal transducer and activator of transcription 3;STAT 3;STAT3

Gene names

STAT3

Research area

Others

Target protein

STAT3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0650Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.249 2.26 2.215
2.271
12ng/ml 1.632 1.599 1.554
1.566
6ng/ml 1.047 1.045 0.999
1.042
3ng/ml 0.776 0.776 0.731
0.776
1.5ng/ml 0.432 0.424 0.379
0.417
0ng/ml 0.056 0.045 0
0.035

Precision

We measured random samples of E0650Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 11.4 0.47 4.1
Intra-Assay 2 18 1.7 0.12 6.8
Intra-Assay 3 18 5.7 0.4 7.1

Protocols

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  • Size

    Code

    Price

  • 96T

    E0650Hu

    $458.00

  • 48T

    E0650Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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