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Home > Product > ELISA Kit > Human Cyclophilin A, CYPA ELISA Kit

Human Cyclophilin A, CYPA ELISA Kit

Human Cyclophilin A, CYPA ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.27ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.27ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cyclophilin A

Code

E0672Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.27ng/ml

Detection range

0.5-200ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the cis-trans isomerization of proline imidic Peptide bonds in oligopeptides (PubMed: 2001362, PubMed: 20676357, PubMed: 21245143, PubMed: 25678563, PubMed: 21593166). Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation (PubMed: 11943775, PubMed: 21245143). Activates endothelial cells (ECs) in a proinflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1 (PubMed: 15130913). Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed: 31063815). In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2 (PubMed: 23180369). Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1 (PubMed: 26095851). Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates (PubMed: 25678563). Plays an important role in platelet activation and aggregation (By similarity). Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton (By similarity). Binds heparan sulfate glycosaminoglycans (PubMed: 11943775). Inhibits replication of influenza A virus (IAV) (PubMed: 19207730). Inhibits ITCH/AIP4-mediated ubiquitination of matrix protein 1 (M1) of IAV by impairing the interaction of ITCH/AIP4 with M1, followed by the suppression of the nuclear export of M1, and finally reduction of the replication of IAV (PubMed: 30328013, PubMed: 22347431). (Microbial infection) May act as a mediator between human SARS coronavirus nucleoprotein and BSG/CD147 in the process of invasion of host cells by the virus (PubMed: 15688292). (Microbial infection) Stimulates RNA-binding ability of HCV NS5A in a peptidyl-prolyl cis-trans isomerase activity-dependent manner.

UniProt accession

MASS(Da)

18,012

GeneID

5478

Synonyms

Cyclophilin A;Cyclosporin A-binding protein;Peptidyl-prolyl cis-trans isomerase A;Peptidyl-prolyl cis-trans isomerase A, N-terminally processed;PPIA;PPIase A;Rotamase A

Gene names

PPIA

Research area

Others

Target protein

PPIA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0672Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/ml 1.965 1.924 1.863
1.882
60ng/ml 0.92 0.971 0.91
1.021
30ng/ml 0.513 0.461 0.4
0.408
15ng/ml 0.229 0.264 0.203
0.298
7.5ng/ml 0.164 0.158 0.098
0.153
0ng/ml 0.062 0.061 0
0.059

Precision

We measured random samples of E0672Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 110.2 2.73 2.5
Intra-Assay 2 18 58.6 2.5 4.3
Intra-Assay 3 18 35.9 1.41 3.9

Protocols

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  • Size

    Code

    Price

  • 96T

    E0672Hu

    $458.00

  • 48T

    E0672Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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