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Home > Product > ELISA Kit > Human Z-DNA Binding Protein 1, ZBP1 ELISA Kit

Human Z-DNA Binding Protein 1, ZBP1 ELISA Kit

Human Z-DNA Binding Protein 1, ZBP1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 7.04ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 7.04ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Z-DNA Binding Protein 1

Code

E0677Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

7.04ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key innate sensor that recognizes and binds Z-RNA structures, which are produced by a number of viruses, such as herpesvirus, orthomyxovirus or flavivirus, and triggers different forms of cell death (PubMed: 32200799). Once activated upon Z-RNA-binding, ZBP1 interacts with RIPK3, inducing the complementary pathways of apoptosis, necroptosis and pyroptosis (By similarity). Acts as a key activator of necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members: ZBP1-dependent necroptosis involves RIPK3 stimulation, which phosphorylates and activates MLKL, triggering execution of programmed necrosis (By similarity). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: ZBP1 recognizes and binds Z-RNA structures that are produced in infected nuclei by orthomyxoviruses, such as the influenza A virus (IAV), leading to ZBP1 activation, RIPK3 stimulation and subsequent MLKL phosphorylation, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (PubMed: 32200799). ZBP1-dependent cell death in response to IAV infection promotes interleukin-1 alpha (IL1A) induction in an NLRP3-inflammasome-independent manner: IL1A expression is required for the optimal interleukin-1 beta (IL1B) production, and together, these cytokines promote infiltration of inflammatory neutrophils to the lung, leading to the formation of neutrophil extracellular traps (By similarity). In some cell types, also able to restrict viral replication by promoting cell death-independent responses (By similarity). In response to Zika virus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with RIPK3, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate (By similarity). Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity).

UniProt accession

MASS(Da)

46,343

GeneID

81030

Synonyms

DAI;DNA-dependent activator of IFN-regulatory factors;Tumor stroma and activated macrophage protein DLM-1;ZBP 1;ZBP1;Z-DNA-binding protein 1

Gene names

ZBP1

Research area

Others

Target protein

ZBP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0677Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.244 2.223 2.164
2.202
800ng/L 1.259 1.362 1.303
1.464
400ng/L 0.677 0.792 0.733
0.907
200ng/L 0.433 0.493 0.434
0.552
100ng/L 0.313 0.304 0.245
0.294
0ng/L 0.068 0.059 0
0.05

Precision

We measured random samples of E0677Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1101.5 33.63 3.1
Intra-Assay 2 18 401.4 26.55 6.6
Intra-Assay 3 18 334.4 19.32 5.8

Protocols

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Associated products

  • Size

    Code

    Price

  • 96T

    E0677Hu

    $458.00

  • 48T

    E0677Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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