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Home > Product > ELISA Kit > Human Forkhead Box P3, FOXP3 ELISA Kit

Human Forkhead Box P3, FOXP3 ELISA Kit

Human Forkhead Box P3, FOXP3 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.09ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.09ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Forkhead Box P3

Code

E0692Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.09ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed: 15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed: 17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed: 17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed: 18368049). Inhibits the transcriptional activator activity of RORA (PubMed: 18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).

UniProt accession

MASS(Da)

47,244

GeneID

50943

Synonyms

Forkhead box protein P3;Forkhead box protein P3 41 kDa form;Forkhead box protein P3, C-terminally processed;FOXP 3;FOXP3;Scurfin

Gene names

FOXP3

Research area

Others

Target protein

FOXP3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0692Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.386 2.33 2.314
2.275
16ng/ml 1.217 1.277 1.261
1.338
8ng/ml 0.574 0.593 0.576
0.612
4ng/ml 0.391 0.391 0.375
0.392
2ng/ml 0.17 0.157 0.141
0.144
0ng/ml 0.014 0.016 0
0.018

Precision

We measured random samples of E0692Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.4 0.4 5.4
Intra-Assay 2 18 12.7 0.6 4.8
Intra-Assay 3 18 21 0.65 3.1

Protocols

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  • Size

    Code

    Price

  • 96T

    E0692Hu

    $458.00

  • 48T

    E0692Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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