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Home > Product > ELISA Kit > Human Matrix Metalloproteinase 14, MMP14 ELISA Kit

Human Matrix Metalloproteinase 14, MMP14 ELISA Kit

Human Matrix Metalloproteinase 14, MMP14 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.62ng/ml

Detection range: 1-400ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.62ng/ml

Detection range: 1-400ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Matrix Metalloproteinase 14

Code

E0714Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.62ng/ml

Detection range

1-400ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity). May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed: 20837484). Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed: 12714657). Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed: 22330140).

UniProt accession

MASS(Da)

65,894

GeneID

4323

Synonyms

Matrix Metalloproteinase-14;Membrane-type Matrix Metalloproteinase 1;Membrane-type-1 Matrix Metalloproteinase;MMP 14;MMP14;MMP-14;MMP-X1;MT1MMP;MT1-MMP;MT-MMP 1;MTMMP1

Gene names

MMP14

Research area

Cardiovascular; Signal Transduction; Cancer; Cell Biology

Target protein

MMP14

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0714Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/ml 2.428 2.353 2.293
2.278
120ng/ml 1.341 1.341 1.281
1.342
60ng/ml 0.862 0.821 0.76
0.779
30ng/ml 0.408 0.408 0.348
0.409
15ng/ml 0.241 0.244 0.184
0.247
0ng/ml 0.078 0.06 0
0.042

Precision

We measured random samples of E0714Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 40.7 2.84 7
Intra-Assay 2 18 58.3 3.3 5.7
Intra-Assay 3 18 166.4 5.92 3.6

Protocols

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  • Size

    Code

    Price

  • 96T

    E0714Hu

    $458.00

  • 48T

    E0714Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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