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Home > Product > ELISA Kit > Human Hexokinase 3, HK3 ELISA Kit

Human Hexokinase 3, HK3 ELISA Kit

Human Hexokinase 3, HK3 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.021ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.021ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Hexokinase 3

Code

E0732Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.021ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed: 8717435). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed: 8717435).

UniProt accession

MASS(Da)

99,025

GeneID

3101

Synonyms

Hexokinase type III;Hexokinase-3;Hexokinase-C;HK 3;HK III;HK3

Gene names

HK3

Research area

Others

Target protein

HK3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0732Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.088 2.107 2.056
2.127
6ng/mL 1.729 1.767 1.716
1.806
3ng/mL 1.095 1.153 1.101
1.211
1.5ng/mL 0.633 0.686 0.634
0.738
0.75ng/mL 0.359 0.392 0.34
0.425
0ng/mL 0.046 0.052 0
0.058

Precision

We measured random samples of E0732Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.4 0.48 7.5
Intra-Assay 2 18 2.3 0.14 6.1
Intra-Assay 3 18 1.5 0.09 5.9

Protocols

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  • Size

    Code

    Price

  • 96T

    E0732Hu

    $458.00

  • 48T

    E0732Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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