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Home > Product > ELISA Kit > Human Mitogen-activated Protein Kinase 6, MAPK6 ELISA Kit

Human Mitogen-activated Protein Kinase 6, MAPK6 ELISA Kit

Human Mitogen-activated Protein Kinase 6, MAPK6 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.092ng/mL

Detection range: 0.2-60ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.092ng/mL

Detection range: 0.2-60ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Mitogen-Activated Protein Kinase 6

Code

E0743Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.092ng/mL

Detection range

0.2-60ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).

UniProt accession

MASS(Da)

82,681

GeneID

5597

Synonyms

ERK-3;Extracellular signal-regulated kinase 3;MAP kinase 6;MAP kinase isoform p97;MAPK 6;MAPK6;Mitogen-activated protein kinase 6;p97-MAPK

Gene names

MAPK6

Research area

Others

Target protein

MAPK6

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0743Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/mL 2.203 2.278 2.22
2.353
16ng/mL 1.41 1.452 1.394
1.493
8ng/mL 0.773 0.781 0.723
0.788
4ng/mL 0.503 0.482 0.424
0.461
2ng/mL 0.272 0.269 0.212
0.267
0ng/mL 0.047 0.058 0
0.068

Precision

We measured random samples of E0743Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 9.2 0.46 5
Intra-Assay 2 18 16 0.53 3.3
Intra-Assay 3 18 5.7 0.35 6.2

Protocols

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  • Size

    Code

    Price

  • 96T

    E0743Hu

    $458.00

  • 48T

    E0743Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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