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Home > Product > ELISA Kit > Human Α1-Antitrypsin, A1-AT ELISA Kit

Human Α1-Antitrypsin, A1-AT ELISA Kit

Human Α1-Antitrypsin, A1-AT ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.024mg/ml

Detection range: 0.05-15mg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.024mg/ml

Detection range: 0.05-15mg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

alpha1-Antitrypsin

Code

E0753Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.024mg/ml

Detection range

0.05-15mg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. [Short Peptide from AAT]: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

UniProt accession

MASS(Da)

46,737

GeneID

5265

Synonyms

Alpha-1 protease inhibitor;Alpha-1-antiproteinase;Alpha-1-antitrypsin;Serpin A1;SERPINA 1;SERPINA1;Short Peptide from AAT;SPAAT

Gene names

SERPINA1

Research area

Cardiovascular

Target protein

SERPINA1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0753Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
9.6mg/ml 1.995 1.942 1.904
1.89
4.8mg/ml 0.917 0.92 0.882
0.923
2.4mg/ml 0.544 0.477 0.438
0.41
1.2mg/ml 0.195 0.212 0.174
0.229
0.6mg/ml 0.077 0.113 0.075
0.149
0mg/ml 0.052 0.038 0
0.025

Precision

We measured random samples of E0753Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6 0.26 4.4
Intra-Assay 2 18 6.9 0.12 1.7
Intra-Assay 3 18 2.6 0.14 5.6

Protocols

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  • Size

    Code

    Price

  • 96T

    E0753Hu

    $458.00

  • 48T

    E0753Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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