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Home > Product > ELISA Kit > Human Map Kinase-activated Protein Kinase 3, MAPKAPK3 ELISA Kit

Human Map Kinase-activated Protein Kinase 3, MAPKAPK3 ELISA Kit

Human Map Kinase-activated Protein Kinase 3, MAPKAPK3 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.027ng/mL

Detection range: 0.05-30ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.027ng/mL

Detection range: 0.05-30ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Map Kinase-Activated Protein Kinase 3

Code

E0759Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.027ng/mL

Detection range

0.05-30ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the Peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.

UniProt accession

MASS(Da)

42,987

GeneID

7867

Synonyms

3pK;Chromosome 3p kinase;MAP kinase-activated protein kinase 3;MAPK-activated protein kinase 3;MAPKAP kinase 3;MAPKAPK 3;MAPKAPK3;MAPKAPK-3;MAPKAP-K3;MK-3

Gene names

MAPKAPK3

Research area

Others

Target protein

MAPKAPK3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0759Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/mL 2.371 2.362 2.319
2.353
8ng/mL 1.56 1.536 1.493
1.511
4ng/mL 1.063 1.129 1.086
1.196
2ng/mL 0.702 0.707 0.664
0.711
1ng/mL 0.425 0.425 0.382
0.425
0ng/mL 0.041 0.043 0
0.045

Precision

We measured random samples of E0759Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.5 0.18 3.3
Intra-Assay 2 18 4.4 0.3 6.8
Intra-Assay 3 18 0.6 0.05 7.1

Protocols

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Associated products

  • Size

    Code

    Price

  • 96T

    E0759Hu

    $458.00

  • 48T

    E0759Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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