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Home > Product > ELISA Kit > Human Indoleamine 2, 3-dioxygenase, IDO ELISA Kit

Human Indoleamine 2, 3-dioxygenase, IDO ELISA Kit

Human Indoleamine 2, 3-dioxygenase, IDO ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Indoleamine 2,3-Dioxygenase

Code

E0796Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.16ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed: 17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed: 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed: 25691885). Acts as a suppressor of anti-tumor immunity (PubMed: 23103127, PubMed: 25157255, PubMed: 14502282, PubMed: 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed: 25691885). Protects the fetus from maternal immune rejection (PubMed: 25691885).

UniProt accession

MASS(Da)

45,326

GeneID

3620

Synonyms

IDO 1;IDO1;IDO-1;Indoleamine 2,3-dioxygenase 1;Indoleamine-pyrrole 2,3-dioxygenase

Gene names

IDO1

Research area

Metabolism

Target protein

IDO1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0796Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.209 2.315 2.289
2.421
24ng/ml 1.404 1.34 1.314
1.275
12ng/ml 0.612 0.585 0.559
0.558
6ng/ml 0.34 0.356 0.33
0.372
3ng/ml 0.181 0.179 0.153
0.176
0ng/ml 0.031 0.026 0
0.021

Precision

We measured random samples of E0796Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 32 0.77 2.4
Intra-Assay 2 18 13.5 0.68 5
Intra-Assay 3 18 12.6 0.59 4.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E0796Hu

    $320.00

  • 96T

    E0796Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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