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Home > Product > ELISA Kit > Human Endothelial Lipase, EL ELISA Kit

Human Endothelial Lipase, EL ELISA Kit

Human Endothelial Lipase, EL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.093ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.093ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Endothelial Lipase

Code

E0933Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.093ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Exerts both phospholipase and triglyceride lipase activities (PubMed: 12032167, PubMed: 10318835, PubMed: 10192396). More active as a phospholipase than a triglyceride lipase (PubMed: 12032167). Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates (PubMed: 12032167). Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins (PubMed: 12032167, PubMed: 10192396).

UniProt accession

MASS(Da)

56,795

GeneID

9388

Synonyms

EDL;EL;Endothelial cell-derived lipase;Endothelial lipase;LIPG;Phospholipase A1

Gene names

LIPG

Research area

Metabolism

Target protein

LIPG

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0933Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.146 2.23 2.191
2.315
20ng/ml 1.26 1.295 1.256
1.33
10ng/ml 0.785 0.774 0.735
0.764
5ng/ml 0.451 0.478 0.439
0.505
2.5ng/ml 0.213 0.195 0.156
0.177
0ng/ml 0.031 0.04 0
0.048

Precision

We measured random samples of E0933Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 26.5 0.83 3.1
Intra-Assay 2 18 9.8 0.59 6
Intra-Assay 3 18 8.3 0.56 6.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E0933Hu

    $320.00

  • 96T

    E0933Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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