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Home > Product > ELISA Kit > Human Histone Deacetylase 8, HDAC8 ELISA Kit

Human Histone Deacetylase 8, HDAC8 ELISA Kit

Human Histone Deacetylase 8, HDAC8 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.055ng/mL

Detection range: 0.1-35ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.055ng/mL

Detection range: 0.1-35ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Histone Deacetylase 8

Code

E0988Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.055ng/mL

Detection range

0.1-35ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility.

UniProt accession

MASS(Da)

41,758

GeneID

55869

Synonyms

HD8;HDAC 8;HDAC8;Histone deacetylase 8

Gene names

HDAC8

Research area

Others

Target protein

HDAC8

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0988Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/mL 2.093 1.969 1.919
1.845
10ng/mL 0.899 1.012 0.962
1.124
5ng/mL 0.505 0.532 0.482
0.56
2.5ng/mL 0.31 0.288 0.238
0.267
1.25ng/mL 0.11 0.114 0.064
0.119
0ng/mL 0.054 0.05 0
0.047

Precision

We measured random samples of E0988Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.5 0.31 4.2
Intra-Assay 2 18 13.1 0.31 2.4
Intra-Assay 3 18 4 0.22 5.4

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E0988Hu

    $320.00

  • 96T

    E0988Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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