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Home > Product > ELISA Kit > Human Androgen Receptor, AR ELISA Kit

Human Androgen Receptor, AR ELISA Kit

Human Androgen Receptor, AR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.095ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.095ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Androgen Receptor

Code

E0991Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.095ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed: 20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

UniProt accession

MASS(Da)

99,188

GeneID

367

Synonyms

Androgen receptor;AR;Dihydrotestosterone receptor;Nuclear receptor subfamily 3 group C member 4

Gene names

AR

Research area

Others

Target protein

AR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0991Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.115 2.096 2.052
2.076
20ng/ml 1.903 1.845 1.801
1.786
10ng/ml 1.264 1.284 1.24
1.304
5ng/ml 0.619 0.617 0.573
0.615
2.5ng/ml 0.43 0.394 0.35
0.359
0ng/ml 0.056 0.044 0
0.032

Precision

We measured random samples of E0991Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.8 0.25 4.2
Intra-Assay 2 18 16.5 1.13 6.8
Intra-Assay 3 18 5.2 0.29 5.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E0991Hu

    $320.00

  • 96T

    E0991Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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