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Home > Product > ELISA Kit > Human Parathyroid Hormone Related Protein, PTHRP ELISA Kit

Human Parathyroid Hormone Related Protein, PTHRP ELISA Kit

Human Parathyroid Hormone Related Protein, PTHRP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.59ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.59ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Parathyroid Hormone Related Protein

Code

E1018Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.59ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Neuroendocrine Peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath (By similarity). Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1-dependent manner through activation of Rac1. Osteostatin is a potent inhibitor of osteoclastic bone resorption.

UniProt accession

MASS(Da)

20,194

GeneID

5744

Synonyms

Osteostatin;Parathyroid hormone-like protein;Parathyroid hormone-related protein;PLP;PTHLH;PTHrP;PTH-rP;PTHrP[107-139];PTHrP[1-36;PTHrP[38-94

Gene names

PTHLH

Research area

Signal Transduction

Target protein

PTHLH

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1018Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 1.796 1.786 1.739
1.775
1000ng/L 1.024 1.005 0.959
0.987
500ng/L 0.445 0.453 0.406
0.46
250ng/L 0.174 0.205 0.158
0.235
125ng/L 0.059 0.08 0.034
0.101
0ng/L 0.035 0.046 0
0.057

Precision

We measured random samples of E1018Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2083 95.02 4.6
Intra-Assay 2 18 1092.4 53.84 4.9
Intra-Assay 3 18 758.6 24.13 3.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E1018Hu

    $320.00

  • 96T

    E1018Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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