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Home > Product > ELISA Kit > Human Hepcidin, HEPC ELISA Kit

Human Hepcidin, HEPC ELISA Kit

Human Hepcidin, HEPC ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.12pg/ml

Detection range: 10-4000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.12pg/ml

Detection range: 10-4000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Hepcidin

Code

E1019Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.12pg/ml

Detection range

10-4000pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed: 22306005). Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed: 11113131, PubMed: 11034317).

UniProt accession

MASS(Da)

9,408

GeneID

57817

Synonyms

HEPC;Hepc20;Hepc25;Hepcidin;Hepcidin-20;Hepcidin-25;LEAP-1;Liver-expressed antimicrobial Peptide 1;PLTR;Putative liver tumor regressor

Gene names

HEPC

Research area

Cardiovascular

Target protein

HEPC

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1019Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400pg/ml 2.091 2.064 2.019
2.038
1200pg/ml 1.289 1.419 1.374
1.549
600pg/ml 0.824 0.883 0.838
0.942
300pg/ml 0.437 0.43 0.385
0.423
150pg/ml 0.225 0.267 0.222
0.308
0pg/ml 0.048 0.045 0
0.042

Precision

We measured random samples of E1019Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 246.2 13.79 5.6
Intra-Assay 2 18 1078.5 47.04 4.4
Intra-Assay 3 18 906.3 50.21 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1019Hu

    $320.00

  • 96T

    E1019Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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