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Home > Product > ELISA Kit > Human Platelet Factor 4, PF-4 ELISA Kit

Human Platelet Factor 4, PF-4 ELISA Kit

Human Platelet Factor 4, PF-4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Platelet Factor 4

Code

E1108Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.025ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.

UniProt accession

MASS(Da)

10,845

GeneID

5196

Synonyms

C-X-C motif chemokine 4;Endothelial cell growth inhibitor;Iroplact;Oncostatin-A;PF 4;PF4;PF-4;Platelet factor 4;Platelet factor 4, short form;

Gene names

PF4

Research area

Cardiovascular

Target protein

PF4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1108Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 1.726 1.77 1.713
1.814
6ng/ml 0.929 1.016 0.96
1.104
3ng/ml 0.568 0.568 0.512
0.569
1.5ng/ml 0.164 0.223 0.166
0.282
0.75ng/ml 0.125 0.11 0.053
0.094
0ng/ml 0.052 0.057 0
0.061

Precision

We measured random samples of E1108Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6 0.2 3.4
Intra-Assay 2 18 7.9 0.24 3.1
Intra-Assay 3 18 4.1 0.21 5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1108Hu

    $320.00

  • 96T

    E1108Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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