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Home > Product > ELISA Kit > Human Platelet Basic Protein, PBP ELISA Kit

Human Platelet Basic Protein, PBP ELISA Kit

Human Platelet Basic Protein, PBP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Platelet Basic Protein

Code

E1135Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.043ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.

UniProt accession

MASS(Da)

13,894

GeneID

5473

Synonyms

Beta-TG;Beta-thromboglobulin;Connective tissue-activating Peptide III;Connective tissue-activating Peptide III(1-81;CTAP-III;CTAP-III(1-81;C-X-C motif chemokine 7;LA-PF4;LDGF;Leukocyte-derived growth factor;Low-affinity platelet factor IV;Macrophage-derived growth factor;MDGF;NAP-2;NAP-2(1-63;NAP-2(1-66;NAP-2(73;NAP-2(74;Neutrophil-activating Peptide 2;Neutrophil-activating Peptide 2(1-63;Neutrophil-activating Peptide 2(1-66;Neutrophil-activating Peptide 2(73;Neutrophil-activating Peptide 2(74;PBP;Platelet basic protein;PPBP;Small-inducible cytokine B7;TC-1;TC-2

Gene names

PPBP

Research area

Immunology

Target protein

PPBP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1135Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.049 2.128 2.082
2.207
12ng/mL 1.373 1.343 1.297
1.314
6ng/mL 0.673 0.679 0.632
0.685
3ng/mL 0.418 0.456 0.409
0.493
1.5ng/mL 0.197 0.194 0.148
0.192
0ng/mL 0.029 0.047 0
0.064

Precision

We measured random samples of E1135Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.4 0.44 5.2
Intra-Assay 2 18 17.7 0.74 4.2
Intra-Assay 3 18 4.1 0.21 5.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E1135Hu

    $320.00

  • 96T

    E1135Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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