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Home > Product > ELISA Kit > Human Plasminogen Activator Inhibitor 1, PAI-1 ELISA Kit

Human Plasminogen Activator Inhibitor 1, PAI-1 ELISA Kit

Human Plasminogen Activator Inhibitor 1, PAI-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Plasminogen Activator Inhibitor 1

Code

E1159Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.019ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed: 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed: 8481516, PubMed: 9207454, PubMed: 17912461). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed: 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed: 15001579, PubMed: 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed: 18386027). It is involved in cellular and replicative senescence (PubMed: 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed: 25808697, PubMed: 27046084).

UniProt accession

MASS(Da)

45,060

GeneID

5054

Synonyms

Endothelial plasminogen activator inhibitor;PAI;PAI-1;Plasminogen activator inhibitor 1;Serpin E1;SERPINE 1;SERPINE1

Gene names

SERPINE1

Research area

Cell Biology

Target protein

SERPINE1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1159Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.07 2.044 1.991
2.018
6ng/ml 1.393 1.367 1.315
1.341
3ng/ml 0.672 0.628 0.575
0.583
1.5ng/ml 0.366 0.338 0.286
0.31
0.75ng/ml 0.179 0.166 0.113
0.152
0ng/ml 0.047 0.053 0
0.058

Precision

We measured random samples of E1159Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3 0.1 3.2
Intra-Assay 2 18 8.1 0.36 4.5
Intra-Assay 3 18 4.7 0.22 4.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E1159Hu

    $320.00

  • 96T

    E1159Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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