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Home > Product > ELISA Kit > Human Thrombomodulin, TM ELISA Kit

Human Thrombomodulin, TM ELISA Kit

Human Thrombomodulin, TM ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Thrombomodulin

Code

E1183Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

UniProt accession

MASS(Da)

60,329

GeneID

7056

Synonyms

CD antigen CD141;Fetomodulin;THBD;Thrombomodulin;TM

Gene names

THBD

Research area

Cardiovascular

Target protein

THBD

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1183Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.192 2.213 2.172
2.235
6ng/ml 1.184 1.24 1.199
1.296
3ng/ml 0.718 0.771 0.729
0.824
1.5ng/ml 0.377 0.415 0.373
0.452
0.75ng/ml 0.223 0.226 0.185
0.23
0ng/ml 0.026 0.042 0
0.058

Precision

We measured random samples of E1183Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.5 0.16 6.6
Intra-Assay 2 18 3.6 0.17 4.6
Intra-Assay 3 18 7.5 0.25 3.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E1183Hu

    $320.00

  • 96T

    E1183Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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