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Home > Product > ELISA Kit > Human Endothelin 1, ET-1 ELISA Kit

Human Endothelin 1, ET-1 ELISA Kit

Human Endothelin 1, ET-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.01ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 1.01ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Endothelin 1

Code

E1238Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.01ng/L

Detection range

2-600ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Endothelins are endothelium-derived vasoconstrictor Peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (PubMed: 19086031).

UniProt accession

MASS(Da)

24,425

GeneID

1906

Synonyms

Big endothelin-1;EDN 1;EDN1;Endothelin-1;ET-1;PPET1;Preproendothelin-1

Gene names

EDN1

Research area

Cancer

Target protein

EDN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1238Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/L 2.504 2.438 2.384
2.372
160ng/L 1.52 1.467 1.413
1.413
80ng/L 0.736 0.778 0.725
0.82
40ng/L 0.439 0.458 0.404
0.477
20ng/L 0.192 0.207 0.153
0.221
0ng/L 0.043 0.053 0
0.063

Precision

We measured random samples of E1238Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 194.2 6.49 3.3
Intra-Assay 2 18 96 4.32 4.5
Intra-Assay 3 18 64.5 3.78 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E1238Hu

    $320.00

  • 96T

    E1238Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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