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Home > Product > ELISA Kit > Human Netrin-1, NTN1 ELISA Kit

Human Netrin-1, NTN1 ELISA Kit

Human Netrin-1, NTN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.39pg/ml

Detection range: 10-3000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.39pg/ml

Detection range: 10-3000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Netrin-1

Code

E1277Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.39pg/ml

Detection range

10-3000pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed: 28483977). Involved in dorsal root ganglion axon projection towards the spinal cord (PubMed: 28483977). It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis (PubMed: 15343335).

UniProt accession

MASS(Da)

67,748

GeneID

9423

Synonyms

Epididymis tissue protein Li 131P;Netrin-1;NTN 1;NTN1

Gene names

NTN1

Research area

Neuroscience

Target protein

NTN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1277Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400pg/ml 2.086 2.058 2.019
2.029
1200pg/ml 1.403 1.4 1.361
1.396
600pg/ml 0.645 0.616 0.578
0.588
300pg/ml 0.367 0.393 0.354
0.419
150pg/ml 0.135 0.157 0.118
0.179
0pg/ml 0.052 0.039 0
0.026

Precision

We measured random samples of E1277Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 693 36.24 5.2
Intra-Assay 2 18 1366.2 48.63 3.6
Intra-Assay 3 18 333.9 13.85 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E1277Hu

    $320.00

  • 96T

    E1277Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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