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Home > Product > ELISA Kit > Human Neuroglobin, NGB ELISA Kit

Human Neuroglobin, NGB ELISA Kit

Human Neuroglobin, NGB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Neuroglobin

Code

E1314Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.26ng/mL

Detection range

0.5-200ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.

UniProt accession

MASS(Da)

16,933

GeneID

58157

Synonyms

Neuroglobin;NGB

Gene names

NGB

Research area

Neuroscience

Target protein

NGB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1314Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/mL 2.038 1.992 1.97
1.946
60ng/mL 1.469 1.452 1.429
1.434
30ng/mL 0.759 0.762 0.739
0.764
15ng/mL 0.453 0.384 0.361
0.314
7.5ng/mL 0.2 0.187 0.164
0.173
0ng/mL 0.025 0.023 0
0.02

Precision

We measured random samples of E1314Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15.2 0.62 4.1
Intra-Assay 2 18 92 5.37 5.8
Intra-Assay 3 18 42.1 2.48 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E1314Hu

    $320.00

  • 96T

    E1314Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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