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Home > Product > ELISA Kit > Human DNA Repair Protein Rad51 Homolog 1, RAD51 ELISA Kit

Human DNA Repair Protein Rad51 Homolog 1, RAD51 ELISA Kit

Human DNA Repair Protein Rad51 Homolog 1, RAD51 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dna Repair Protein Rad51 Homolog 1

Code

E1370Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.11ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed: 28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed: 26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed: 26253028).

UniProt accession

MASS(Da)

36,966

GeneID

5888

Synonyms

DNA repair protein RAD51 homolog 1;hRAD51;HsRAD51;RAD51;RAD51 homolog A

Gene names

RAD51

Research area

Others

Target protein

RAD51

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1370Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.546 2.538 2.482
2.531
20ng/ml 1.467 1.48 1.423
1.492
10ng/ml 0.904 0.859 0.802
0.814
5ng/ml 0.386 0.419 0.363
0.453
2.5ng/ml 0.265 0.23 0.173
0.195
0ng/ml 0.057 0.057 0
0.056

Precision

We measured random samples of E1370Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 17.1 0.5 2.9
Intra-Assay 2 18 10.1 0.46 4.6
Intra-Assay 3 18 7.6 0.42 5.5

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1370Hu

    $320.00

  • 96T

    E1370Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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