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Home > Product > ELISA Kit > Human Glypican-3, GPC-3 ELISA Kit

Human Glypican-3, GPC-3 ELISA Kit

Human Glypican-3, GPC-3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Glypican-3

Code

E1431Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.01ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cell surface proteoglycan that bears heparan sulfate (PubMed: 14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed: 16227623, PubMed: 24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed: 17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity).

UniProt accession

MASS(Da)

65,563

GeneID

2719

Synonyms

Glypican-3;Glypican-3 alpha subunit;Glypican-3 beta subunit;GPC 3;GPC3;GTR2-2;Intestinal protein OCI-5;MXR7

Gene names

GPC3

Research area

Signal Transduction

Target protein

GPC3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1431Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.278 2.261 2.223
2.245
3.2ng/ml 1.397 1.303 1.264
1.208
1.6ng/ml 0.835 0.849 0.81
0.863
0.8ng/ml 0.384 0.434 0.395
0.483
0.4ng/ml 0.237 0.246 0.207
0.254
0ng/ml 0.037 0.039 0
0.04

Precision

We measured random samples of E1431Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.7 0.14 3.9
Intra-Assay 2 18 1.1 0.07 6.7
Intra-Assay 3 18 1.9 0.1 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1431Hu

    $320.00

  • 96T

    E1431Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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