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Home > Product > ELISA Kit > Human Thioredoxin, TRX ELISA Kit

Human Thioredoxin, TRX ELISA Kit

Human Thioredoxin, TRX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.49U/L

Detection range: 5-1500U/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.49U/L

Detection range: 5-1500U/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Thioredoxin

Code

E1452Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.49U/L

Detection range

5-1500U/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed: 2176490, PubMed: 17182577, PubMed: 19032234). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed: 16408020, PubMed: 17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed: 9108029, PubMed: 11118054). ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

UniProt accession

MASS(Da)

11,737

GeneID

7295

Synonyms

ADF;allergen Hom s Trx;ATL-derived factor;SASP;Surface-associated sulphydryl protein;Thioredoxin;Trx;TXN

Gene names

TXN

Research area

Metabolism

Target protein

TXN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1452Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800U/L 2.153 2.143 2.085
2.134
400U/L 0.994 1.015 0.956
1.036
200U/L 0.496 0.482 0.423
0.467
100U/L 0.217 0.192 0.134
0.168
50U/L 0.104 0.12 0.061
0.136
0U/L 0.056 0.058 0
0.061

Precision

We measured random samples of E1452Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 161.6 7.43 4.6
Intra-Assay 2 18 605.8 13.7 2.3
Intra-Assay 3 18 311.7 10.89 3.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1452Hu

    $320.00

  • 96T

    E1452Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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