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Home > Product > ELISA Kit > Human Lipoprotein Lipase, LPL ELISA Kit

Human Lipoprotein Lipase, LPL ELISA Kit

Human Lipoprotein Lipase, LPL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.49pg/ml

Detection range: 10-4000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.49pg/ml

Detection range: 10-4000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Lipoprotein Lipase

Code

E1517Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.49pg/ml

Detection range

10-4000pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed: 8675619, PubMed: 11342582, PubMed: 27578112). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed: 7592706, PubMed: 12032167). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed: 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed: 11342582, PubMed: 27811232).

UniProt accession

MASS(Da)

53,162

GeneID

4023

Synonyms

Lipoprotein lipase;LPL;Phospholipase A1

Gene names

LPL

Research area

Signal Transduction

Target protein

LPL

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1517Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400pg/ml 2.121 2.128 2.079
2.136
1200pg/ml 1.479 1.405 1.355
1.33
600pg/ml 0.724 0.833 0.783
0.941
300pg/ml 0.443 0.461 0.412
0.48
150pg/ml 0.223 0.226 0.177
0.23
0pg/ml 0.047 0.05 0
0.053

Precision

We measured random samples of E1517Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1156.6 48.6 4.2
Intra-Assay 2 18 322.8 19.48 6
Intra-Assay 3 18 672 23.66 3.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1517Hu

    $320.00

  • 96T

    E1517Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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