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Home > Product > ELISA Kit > Human Osteopontin, OPN ELISA Kit

Human Osteopontin, OPN ELISA Kit

Human Osteopontin, OPN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.15ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.15ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Osteopontin

Code

E1525Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.15ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

UniProt accession

MASS(Da)

35,423

GeneID

6696

Synonyms

Bone sialoprotein 1;Nephropontin;Osteopontin;Secreted phosphoprotein 1;SPP 1;SPP1;SPP-1;Urinary stone protein;Uropontin;

Gene names

SPP1

Research area

Cancer

Target protein

SPP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1525Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 1.978 1.908 1.868
1.839
24ng/ml 0.996 1.045 1.005
1.094
12ng/ml 0.44 0.448 0.408
0.456
6ng/ml 0.28 0.253 0.214
0.227
3ng/ml 0.129 0.103 0.063
0.077
0ng/ml 0.053 0.04 0
0.026

Precision

We measured random samples of E1525Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 43.8 1.49 3.4
Intra-Assay 2 18 15.3 0.65 4.2
Intra-Assay 3 18 26.2 1.2 4.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E1525Hu

    $320.00

  • 96T

    E1525Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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