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Home > Product > ELISA Kit > Human Myelin Basic Protein, MBP ELISA Kit

Human Myelin Basic Protein, MBP ELISA Kit

Human Myelin Basic Protein, MBP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 32.27ng/L

Detection range: 50-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 32.27ng/L

Detection range: 50-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Myelin Basic Protein

Code

E1531Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

32.27ng/L

Detection range

50-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

UniProt accession

MASS(Da)

33,117

GeneID

4155

Synonyms

MBP;Myelin A1 protein;Myelin basic protein;Myelin membrane encephalitogenic protein

Gene names

MBP

Research area

Neuroscience

Target protein

MBP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1531Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 1.664 1.683 1.637
1.703
2000ng/L 0.98 0.966 0.92
0.952
1000ng/L 0.501 0.457 0.41
0.413
500ng/L 0.138 0.189 0.142
0.239
250ng/L 0.085 0.098 0.052
0.111
0ng/L 0.034 0.046 0
0.058

Precision

We measured random samples of E1531Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2110.5 94.49 4.5
Intra-Assay 2 18 3193.5 127.66 4
Intra-Assay 3 18 1308.1 56.27 4.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E1531Hu

    $320.00

  • 96T

    E1531Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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