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Home > Product > ELISA Kit > Human Apolipoprotein B100, APO-B100 ELISA Kit

Human Apolipoprotein B100, APO-B100 ELISA Kit

Human Apolipoprotein B100, APO-B100 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.01mg/ml

Detection range: 0.03-6mg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.01mg/ml

Detection range: 0.03-6mg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Apolipoprotein B100

Code

E1536Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.01mg/ml

Detection range

0.03-6mg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

UniProt accession

MASS(Da)

515,605

GeneID

338

Synonyms

Apo B-100;Apo B-48;APOB;Apolipoprotein B-100;Apolipoprotein B-48

Gene names

APOB

Research area

Cardiovascular; Signal Transduction

Target protein

APOB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1536Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3.2mg/ml 2.646 2.626 2.579
2.607
1.6mg/ml 1.547 1.546 1.498
1.544
0.8mg/ml 0.869 0.806 0.758
0.742
0.4mg/ml 0.531 0.47 0.423
0.41
0.2mg/ml 0.217 0.212 0.164
0.207
0mg/ml 0.046 0.048 0
0.049

Precision

We measured random samples of E1536Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.4 0.04 2.9
Intra-Assay 2 18 0.9 0.05 5.4
Intra-Assay 3 18 0.4 0.02 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E1536Hu

    $320.00

  • 96T

    E1536Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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