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Home > Product > ELISA Kit > Human Semaphorin 3E, SEMA3E ELISA Kit

Human Semaphorin 3E, SEMA3E ELISA Kit

Human Semaphorin 3E, SEMA3E ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.39ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.39ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Semaphorin 3E

Code

E1562Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.39ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation (By similarity).

UniProt accession

MASS(Da)

89,228

GeneID

9723

Synonyms

SEMA3E;Semaphorin-3E

Gene names

SEMA3E

Research area

Others

Target protein

SEMA3E

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1562Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.12 2.236 2.199
2.352
800ng/L 1.661 1.709 1.672
1.756
400ng/L 1.179 1.14 1.104
1.101
200ng/L 0.727 0.713 0.677
0.7
100ng/L 0.471 0.443 0.406
0.414
0ng/L 0.047 0.037 0
0.026

Precision

We measured random samples of E1562Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 288.3 17.85 6.2
Intra-Assay 2 18 557.8 28.35 5.1
Intra-Assay 3 18 116 7.75 6.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E1562Hu

    $320.00

  • 96T

    E1562Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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