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Home > Product > ELISA Kit > Human PIgMent Epithelium-derived Factor, PEDF ELISA Kit

Human PIgMent Epithelium-derived Factor, PEDF ELISA Kit

Human PIgMent Epithelium-derived Factor, PEDF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.017ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.017ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Pigment Epithelium-Derived Factor

Code

E1634Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.017ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

UniProt accession

MASS(Da)

46,312

GeneID

5176

Synonyms

Cell proliferation-inducing gene 35 protein;EPC-1;PEDF;Pigment epithelium-derived factor;Serpin F1;SERPINF 1;SERPINF1

Gene names

SERPINF1

Research area

Cancer

Target protein

SERPINF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1634Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.117 2.149 2.105
2.181
6ng/ml 1.238 1.319 1.274
1.4
3ng/ml 0.656 0.692 0.647
0.728
1.5ng/ml 0.311 0.372 0.328
0.433
0.75ng/ml 0.119 0.141 0.096
0.162
0ng/ml 0.045 0.044 0
0.043

Precision

We measured random samples of E1634Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.4 0.21 4.9
Intra-Assay 2 18 7.4 0.27 3.6
Intra-Assay 3 18 3.1 0.18 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E1634Hu

    $320.00

  • 96T

    E1634Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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