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Home > Product > ELISA Kit > Human Vimentin, VIM ELISA Kit

Human Vimentin, VIM ELISA Kit

Human Vimentin, VIM ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.07ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.07ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Vimentin

Code

E1673Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.07ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

UniProt accession

MASS(Da)

53,652

GeneID

7431

Synonyms

VIM;Vimentin

Gene names

VIM

Research area

Cancer

Target protein

VIM

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1673Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.01 2.009 1.954
2.008
16ng/ml 1.387 1.366 1.312
1.346
8ng/ml 0.599 0.587 0.532
0.575
4ng/ml 0.458 0.409 0.354
0.36
2ng/ml 0.192 0.159 0.105
0.126
0ng/ml 0.059 0.055 0
0.051

Precision

We measured random samples of E1673Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 24 1.2 5
Intra-Assay 2 18 10.7 0.48 4.5
Intra-Assay 3 18 4.6 0.22 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E1673Hu

    $320.00

  • 96T

    E1673Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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