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Home > Product > ELISA Kit > Human Atp-sensitive Inward Rectifier Potassium Channel 1, KCNJ1 ELISA Kit

Human Atp-sensitive Inward Rectifier Potassium Channel 1, KCNJ1 ELISA Kit

Human Atp-sensitive Inward Rectifier Potassium Channel 1, KCNJ1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.017ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.017ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Atp-Sensitive Inward Rectifier Potassium Channel 1

Code

E1792Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.017ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium Channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the Channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This Channel is activated by internal ATP and can be blocked by external barium.

UniProt accession

MASS(Da)

44,795

GeneID

3758

Synonyms

ATP-regulated potassium Channel ROM-K;ATP-sensitive inward rectifier potassium Channel 1;Inward rectifier K(+ Channel Kir1.1;KCNJ 1;KCNJ1;Potassium Channel, inwardly rectifying subfamily J member 1

Gene names

KCNJ1

Research area

Others

Target protein

KCNJ1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1792Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.303 2.162 2.101
2.022
4ng/ml 1.421 1.488 1.427
1.556
2ng/ml 0.767 0.773 0.711
0.779
1ng/ml 0.526 0.528 0.467
0.531
0.5ng/ml 0.242 0.271 0.209
0.3
0ng/ml 0.066 0.061 0
0.056

Precision

We measured random samples of E1792Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.8 0.1 5.6
Intra-Assay 2 18 4.9 0.2 4.1
Intra-Assay 3 18 0.8 0.04 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E1792Hu

    $320.00

  • 96T

    E1792Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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