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Home > Product > ELISA Kit > Bovine Osteopontin, OPN ELISA Kit

Bovine Osteopontin, OPN ELISA Kit

Bovine Osteopontin, OPN ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 3.26ng/ml

Detection range: 7-1500ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 3.26ng/ml

Detection range: 7-1500ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Osteopontin

Code

E0402Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

3.26ng/ml

Detection range

7-1500ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Major non-collagenous bone protein that binds tightly to hydroxyapatite (Probable). Appears to form an integral part of the mineralized matrix (Probable). Probably important to cell-matrix interaction (Probable). Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

UniProt accession

MASS(Da)

30,904

GeneID

281499

Synonyms

Bone sialoprotein 1;BSPI;ETA1;OPN;Osteopontin;Secreted phosphoprotein 1;SPP 1;SPP1;SPP-1;

Gene names

SPP1

Research area

Cardiovascular; Signal Transduction

Target protein

OPN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0402Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/ml 2.087 2.063 2.016
2.038
400ng/ml 1.641 1.677 1.63
1.712
200ng/ml 1.149 1.065 1.017
0.981
100ng/ml 0.633 0.629 0.582
0.625
50ng/ml 0.484 0.442 0.395
0.401
0ng/ml 0.06 0.047 0
0.034

Precision

We measured random samples of E0402Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 256.2 12.19 4.8
Intra-Assay 2 18 78.8 3.75 4.8
Intra-Assay 3 18 209.2 12.45 6

Protocols

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  • Size

    Code

    Price

  • 96T

    E0402Bo

    $458.00

  • 48T

    E0402Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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